A novel 3D model to study the link between hormonal exposure and mammographic density in breast cancer

• Lower type 1 collagen concentra1on (0.5 mg/ml) increases elonga1on of structures compared to 1 mg/ml.
• Fibroblasts enhances elonga1on of structures in 1 mg/ml, but not 0.5 mg/ml.
• In 1 mg/ml collagen, elongated epithelial structures had lumen forma1on when co-­‐cultured with fibroblasts.
• Hormones in co-­‐cultures of T47D + fibroblasts, significantly altered the phenotype in terms of elonga1on.